The using of gamma-octanolactone in the broiler diet: intestinal microbiome

It is known that the inclusion of antibiotics in chicken feed leads to development resistance bacteria. for this reason search additives alternative becomes relevant. The ability gamma-glucanolactone inhibit various quorum systems (QS) LuxI/LuxR bacteria has been demonstrated. Based on this, it can be assumed use substance diet will reduce infections intestines poultry and improve effects broiler rearing. aim work was evaluate microbiome iliac intestine broilers when introducing gammaoctanolactone into diet.The study conducted using sequencing method. Total DNA from samples intestinal contents isolated FastDNA ® Faecal Extraction Kit. Pair-end library amplicons performed Illumina MiSeq Reagent Kit platform v.2 (500 cycles) (Illumina, San Diego, California, USA). gamma-octalactone at a dosage 0.05 0,20 ml / kg w.m. day contributes an increase number representatives phylum Firmicutes poultry. 0,05 gamma-octanolactone reduced content genus Streptococcus by 4,21%; w.m./ increased 10% genera Lactobacillus Turicibacter, relative control.